The fragments z1 br15 to z4 br15 were amplified by pcr from their respective plasmids using a set of primer pairs that were designed so that z1 br15 and z2 br15 or z3 br15 and z4 br15 matched on about 30 40.
Z2 m el.
After 6 days the entire contents of the well was trypsinized and counted using a beckman z2 coulter counter with a size selection setting of 8 30 μm.
Cybrids or 143b ρ0 cells were plated in triplicate in 12 well plates at 5 000 20 000 cells per well in 1 5 ml as described in each experiment.
A cytomegalovirus cmv promoter sequence driving the expression of viral fragment from nucleotides 1 2252 of genomic rna was included in z1.
A bell curve is a graph depicting the normal distribution which has a shape reminiscent of a bell.
Open table in a new tab the results of principal component analysis and least square fitting of linear components of near edge as k bulk xas conducted on fresh flash frozen leaves and roots of eir1 1 pin2 and wild type demonstrated that the majority of the.
Z2 denotes the area of transition zone plus root apical meristems.
The synthetic genes z1 br15 z2 br15 z3 br15 and z4 br15 were synthesized and cloned into plasmid puc57 by genecust luxembourg.
The top of the curve shows the mean mode and median of the data collected.
The three other synthetic genes comprised the portions of the genome coding for the nonstructural proteins from nucleotides 2210 5616 z2 5577 8201 z3 and 8167 10 801 z4 fig.